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Proceedings Article

Analysis of cellular structure and dynamics with digital holographic microscopy

[+] Author Affiliations
P. Marquet

Ctr. de Neurosciences Psychiatriques, CHUV (Switzerland)

B. Rappaz

Brain Mind Institute, EPFL (Switzerland)

F. Charrière

Imaging and Applied Optics Institute, EPFL (Switzerland)

Y. Emery

Lyncée Tec SA, PSE-A (Switzerland)

C. Depeursinge

Ecole Polytechnique Fédérale de Lausanne (Switzerland)

P. Magistretti

Ctr. de Neurosciences Psychiatriques, CHUV (Switzerland) and Brain Mind Institute, EPFL (Switzerland)

Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330F (July 13, 2007); doi:10.1117/12.738579
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From Conference Volume 6633

  • Biophotonics 2007: Optics in Life Science
  • Jürgen Popp; Gert von Bally
  • Munich, Germany | June 17, 2007

abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

P. Marquet ; B. Rappaz ; F. Charrière ; Y. Emery ; C. Depeursinge, et al.
"Analysis of cellular structure and dynamics with digital holographic microscopy", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330F (July 13, 2007); doi:10.1117/12.738579; http://dx.doi.org/10.1117/12.738579


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