Optimal excitation wavelengths for discrimination of nasopharyngeal carcinoma in vitro

Lisheng Lin; Fuwen Yang; Buhong Li; Shusen Xie

doi:10.1117/12.756009

8 January 2008 Optimal excitation wavelengths for discrimination of nasopharyngeal carcinoma in vitro

Lisheng Lin, Fuwen Yang, Buhong Li, Shusen Xie

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68262Y (2008) https://doi.org/10.1117/12.756009
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Measurement of excitation-emission matrices (EEMs) for nasopharyngeal carcinoma (NPC) and normal nasopharyngeal tissues in vitro was employed to determine the optimal excitation wavelengths that contain the most diagnostic information for optical diagnosis. Pathologically confirmed normal nasopharyngeal and NPC tissues(n=47) were obtained from 25 patients undergoing surgical biopsy. The autofluorescence EEMs of tissue biopsies were recorded with a TCSPC spectrofluorimeter. Fluorescence excitation wavelengths varied from 260 to 500 nm, and the corresponding fluorescence emission spectra were recorded from a range starting 20 nm above the excitation wavelength and extending to 700 nm. The calibration for different EEMs measurements was performed for quantitative comparison. As a result, we find that the excitation-emission pair of 340-380 nm yields the best performance for discrimination of NPC tissues with the sensitivity and specificity of 77.2% and 88.0%, respectively

Citation Download Citation

Lisheng Lin, Fuwen Yang, Buhong Li, and Shusen Xie "Optimal excitation wavelengths for discrimination of nasopharyngeal carcinoma in vitro", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262Y (8 January 2008); https://doi.org/10.1117/12.756009

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