In vivo endoscopic autofluorescence microspectro-imaging of bronchi and alveoli

G. Bourg-Heckly; L. Thiberville M.D.; C. Vever-Bizet; B. Viellerobe

doi:10.1117/12.762211

21 February 2008 In vivo endoscopic autofluorescence microspectro-imaging of bronchi and alveoli

G. Bourg-Heckly, L. Thiberville M.D., C. Vever-Bizet, B. Viellerobe

Proceedings Volume 6851, Endoscopic Microscopy III; 685104 (2008) https://doi.org/10.1117/12.762211
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Fibered confocal fluorescence microscopy (FCFM) is an emerging technique that can be used during bronchoscopy to analyze the nature of the human bronchial mucosa and alveolar network fluorescence microstructure. An endoscopic fibered confocal fluorescence microscopy system with spectroscopic analysis capability was developed allowing realtime, simultaneous images and emission spectra acquisition, at 488 nm excitation, using a flexible miniprobe. This flexible 1.4 mm miniprobe can be introduced into the working channel of a flexible endoscope and gently advanced through the bronchial tree up to the alveoli. FCFM in conjunction with bronchoscopy is able to image the in vivo autofluorescence microstructure of the bronchial mucosa but also the alveolar respiratory network outside of the usual field of view. In the normal bronchi, reproducible images were obtained, characterized by a highly organized fibered network. Precancerous lesions exhibited alterations of this fibered network. Microscopic and spectral analysis showed that the signal mainly originates from the elastin component of the bronchial subepithelial layer. In non smokers, the system images the elastin backbone of the aveoli. In active smokers, a strong autofluorescence signal appears from alveolar macrophages. The FCFM technique appears promising for in vivo exploration of the bronchial and alveolar extracellular matrix.

Citation Download Citation

G. Bourg-Heckly, L. Thiberville M.D., C. Vever-Bizet, and B. Viellerobe "In vivo endoscopic autofluorescence microspectro-imaging of bronchi and alveoli", Proc. SPIE 6851, Endoscopic Microscopy III, 685104 (21 February 2008); https://doi.org/10.1117/12.762211

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