Combined use of fluorescent and dynamic light scattering imaging for applications in vascular biology

Vyacheslav Kalchenko M.D.; Keren Ziv; Yoseph Addadi; Michal Neeman; Alon Harmelin D.V.M.

doi:10.1117/12.763457

6 February 2008 Combined use of fluorescent and dynamic light scattering imaging for applications in vascular biology

Vyacheslav Kalchenko M.D., Keren Ziv, Yoseph Addadi, Michal Neeman, Alon Harmelin D.V.M.

Proceedings Volume 6855, Complex Dynamics and Fluctuations in Biomedical Photonics V; 68550D (2008) https://doi.org/10.1117/12.763457
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We present an application of custom-designed Dynamic Light Scattering Imager (DLSI) in combination with conventional fluorescence intravital microscope (FIVM). The proposed technology was used for simultaneous examination of blood and lymphatic vessels in the mouse ear or tumor development. DLSI comprised a 650 nm diode laser with beam expander. Temporal fluctuations of laser interference pattern were used for rendering blood vessels anatomy and perfusion with a high spatial resolution. Concomitantly, various fluorescent contrast materials were used for labeling and visualization of lymphatic vessels or the tumor cells. The modular design of FIVM-DLSI allowed easy switching between different models of microscopes while conventional image sensors could be employed for both fluorescence and DLS imaging. We demonstrated that coupling with DLSI expands the fluorescent microscope imaging capabilities and does not compromise its ability to image with a high spatial resolution.

Citation Download Citation

Vyacheslav Kalchenko M.D., Keren Ziv, Yoseph Addadi, Michal Neeman, and Alon Harmelin D.V.M. "Combined use of fluorescent and dynamic light scattering imaging for applications in vascular biology", Proc. SPIE 6855, Complex Dynamics and Fluctuations in Biomedical Photonics V, 68550D (6 February 2008); https://doi.org/10.1117/12.763457

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