Monitoring the effect of mechanical stress on mesenchymal stem cell collagen production by multiphoton microscopy

Wei-Liang Chen; Chia-Cheng Chang; Ling-Ling Chiou; Tsung-Hsien Li; Yuan Liu; Hsuan-Shu Lee M.D.; Chen-Yuan Dong

doi:10.1117/12.763227

6 February 2008 Monitoring the effect of mechanical stress on mesenchymal stem cell collagen production by multiphoton microscopy

Wei-Liang Chen, Chia-Cheng Chang, Ling-Ling Chiou, Tsung-Hsien Li, Yuan Liu, Hsuan-Shu Lee M.D., Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6858, Optics in Tissue Engineering and Regenerative Medicine II; 685806 (2008) https://doi.org/10.1117/12.763227
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Tissue engineering is emerging as a promising method for repairing damaged tissues. Due to cartilage's common wear and injury, in vitro production of cartilage replacements have been an active area of research. Finding the optimal condition for the generation of the collagen matrix is crucial in reproducing cartilages that closely match those found in human. Using multiphoton autofluorescence and second-harmonic generation (SHG) microscopy we monitored the effect of mechanical stress on mesenchymal stem cell collagen production. Bone marrow mesenchymal stem cells in the form of pellets were cultured and periodically placed under different mechanical stress by centrifugation over a period of four weeks. The differently stressed samples were imaged several times during the four week period, and the collagen production under different mechanical stress is characterized.

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Wei-Liang Chen, Chia-Cheng Chang, Ling-Ling Chiou, Tsung-Hsien Li, Yuan Liu, Hsuan-Shu Lee M.D., and Chen-Yuan Dong "Monitoring the effect of mechanical stress on mesenchymal stem cell collagen production by multiphoton microscopy", Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 685806 (6 February 2008); https://doi.org/10.1117/12.763227

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