Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Advantages of digital holographic microscopy for real-time full field absolute phase imaging

[+] Author Affiliations
Tristan Colomb

Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland) and Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Florian Charrière, Jonas Kühn, Christian Depeursinge

Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Pierre Marquet

Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)

Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686109 (February 12, 2008); doi:10.1117/12.763284
Text Size: A A A
From Conference Volume 6861

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Jose, CA | January 19, 2008

abstract

Different interferometric techniques were developed last decade to obtain full field, quantitative, and absolute phase imaging, such as phase-shifting, Fourier phase microscopy, Hilbert phase microscopy or digital holographic microscopy (DHM). Although, these techniques are very similar, DHM combines several advantages. In contrast, to phase shifting, DHM is indeed capable of single-shot hologram recording allowing a real-time absolute phase imaging. On the other hand, unlike to Fourier phase or Hilbert phase microscopy, DHM does not require to record in focus images of the specimen on the digital detector (CCD or CMOS camera), because a numerical focalization adjustment can be performed by a numerical wavefront propagation. Consequently, the depth of view of high NA microscope objectives is numerically extended. For example, two different biological cells, floating at different depths in a liquid, can be focalized numerically from the same digital hologram. Moreover, the numerical propagation associated to digital optics and automatic fitting procedures, permits vibrations insensitive full- field phase imaging and the complete compensation for a priori any image distortion or/and phase aberrations introduced for example by imperfections of holders or perfusion chamber. Examples of real-time full-field phase images of biological cells have been demonstrated.

© (2008) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Tristan Colomb ; Florian Charrière ; Jonas Kühn ; Pierre Marquet and Christian Depeursinge
"Advantages of digital holographic microscopy for real-time full field absolute phase imaging", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686109 (February 12, 2008); doi:10.1117/12.763284; http://dx.doi.org/10.1117/12.763284


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.