Identification of antibody isotypes in biological fluids by means of micro-Raman spectroscopy and chemometric methods

C. Araujo-Andrade; J. L. Pichardo-Molina; G. Barbosa-Sabanero; C. Frausto-Reyes

doi:10.1117/12.761978

22 February 2008 Identification of antibody isotypes in biological fluids by means of micro-Raman spectroscopy and chemometric methods

C. Araujo-Andrade, J. L. Pichardo-Molina, G. Barbosa-Sabanero, C. Frausto-Reyes

Author Affiliations +

Proceedings Volume 6863, Optical Diagnostics and Sensing VIII; 68630P (2008) https://doi.org/10.1117/12.761978
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Clinical diagnosis of infections, generally are realized by serological methods, which identifies the antibodies presents in serum or tissue fluids of the patient. Antibodies are proteins present in our bodies that aid in the elimination of pathogens or antigens. Identification of antibodies isotypes is important because can help to predict when and whether patients will recover from infections and are commonly diagnosed by means of indirect methods such as serological test. In the other hand, the majority of these methods requires specific kits for the analysis, special sample preparation, chemical reagents, expensive equipment and require long time for getting results. In this work we show the feasibility to discriminate antibody isotypes in biological fluids like human colostrum by means of Raman spectroscopy and chemometrics. Spectra were obtained using an excitation wavelength of 514 nm over dried samples of human colostrum labeled previously as positives to specific IgG and IgM antibodies against Toxoplasma Gondii by means of ELISA test. Partial least square-discriminant analysis (PLS-DA) was used to discriminate among antibody isotypes by use second derivative of Raman spectra of colostrum samples.

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C. Araujo-Andrade, J. L. Pichardo-Molina, G. Barbosa-Sabanero, and C. Frausto-Reyes "Identification of antibody isotypes in biological fluids by means of micro-Raman spectroscopy and chemometric methods", Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 68630P (22 February 2008); https://doi.org/10.1117/12.761978

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KEYWORDS

Raman spectroscopy

Statistical analysis

Biological research

Chemical analysis

Chemometrics

Microfluidics

Analytical research

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