Quantum dots-based probes conjugated to Annexin V for photostable apoptosis detection and imaging

Séverine Le Gac; Istvan Vermes; Albert van den Berg

doi:10.1117/12.787662

22 February 2008 Quantum dots-based probes conjugated to Annexin V for photostable apoptosis detection and imaging

Séverine Le Gac, Istvan Vermes, Albert van den Berg

Proceedings Volume 6866, Colloidal Quantum Dots for Biomedical Applications III; 68660I (2008) https://doi.org/10.1117/12.787662
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Quantum dots (Qdots) are nanoparticles exhibiting fluorescent properties that are widely applied for cell staining. We present here the development of quantum dots for specific targeting of apoptotic cells, for both apoptosis detection and staining of apoptotic "living" cells. These Qdots are functionalized with Annexin V, a 35-kDa protein that specifically interacts with the membrane of apoptotic cells: Annexin V recognizes and binds to phosphatidylserine (PS) moieties which are present on the outer membrane of apoptotic cells and not on this of healthy or necrotic cells. By using Annexin V, our Qdots probes are made specific for apoptotic cells. For that purpose, Qdots Streptavidin Conjugates are coupled to biotinylated Annexin V. Staining of apoptotic cells was checked using fluorescence and confocal microscopy techniques on nonfixed cells. It is shown here that Qdots are insensitive to bleaching after prolonged and frequent exposure as opposed to organic dyes and this makes them excellent candidates for time-lapse imaging purposes. We illustrate the application of our Qdots-based probes to continuously follow fast changes occurring on the membrane of apoptotic cells.

Citation Download Citation

Séverine Le Gac, Istvan Vermes, and Albert van den Berg "Quantum dots-based probes conjugated to Annexin V for photostable apoptosis detection and imaging", Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660I (22 February 2008); https://doi.org/10.1117/12.787662

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