Optimizing protocols for imaging neural cells and tissues using functionalized quantum dots

Smita Pathak; Gabriel A. Silva

doi:10.1117/12.784337

26 February 2008 Optimizing protocols for imaging neural cells and tissues using functionalized quantum dots

Smita Pathak, Gabriel A. Silva

Proceedings Volume 6866, Colloidal Quantum Dots for Biomedical Applications III; 68660V (2008) https://doi.org/10.1117/12.784337
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Chemically functionalized semiconductor quantum dot protocols were optimized for the specific labeling and imaging of neural cells, both neurons and macroglial cells. Beta-tubulin III was used to image primary cortical neurons and PC12 cells while glial fibrillary acidic protein (GFAP) was used to image primary spinal cord and cortical astrocytes and the rMC-1 retinal glial Muller cell line. Both proteins are the main components of intermediate filaments and are specific to the two classes of neural cells. We also specifically labeled and imaged at high resolutions using anti-GFAP conjugated quantum dots glial scars in situ in intact neural sensory retina in a rodent model of macular degeneration.

Citation Download Citation

Smita Pathak and Gabriel A. Silva "Optimizing protocols for imaging neural cells and tissues using functionalized quantum dots", Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660V (26 February 2008); https://doi.org/10.1117/12.784337

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