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Proceedings Article

Teal fluorescent proteins: characterization of a reversibly photoswitchable variant

[+] Author Affiliations
Hui-wang Ai, Robert E. Campbell

Univ. of Alberta (Canada)

Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680D (February 13, 2008); doi:10.1117/12.761423
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From Conference Volume 6868

  • Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes
  • Alexander P. Savitsky; Robert E. Campbell; Robert M. Hoffman
  • San Jose, CA | January 19, 2008

abstract

Fluorescent proteins (FPs) emerged in the mid 1990s as a powerful tool for life science research. Cyan FPs (CFPs), widely used in multicolor imaging or as a fluorescence resonance energy transfer (FRET) donor to yellow FPs (YFPs), were considerably less optimal than other FPs because of some relatively poor photophysical properties. We recently initiated an effort to create improved or alternate versions of CFPs. To address the limitations of CFPs, an alternative known as monomeric teal FP1 (mTFP1) was engineered from the naturally tetrameric Clavularia CFP, by screening either rationally designed or random libraries of variants. mTFP1 has proven to be a particularly useful new member of the FP 'toolbox' by facilitating multicolor live cell imaging. During the directed evolution process of mTFP1, it was noticed that some earlier variants underwent fast and reversible photoisomerization. Some of the initial characterization of one particular mutant, designated as mTFP0.7, is described in this manuscript.

© (2008) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Hui-wang Ai and Robert E. Campbell
"Teal fluorescent proteins: characterization of a reversibly photoswitchable variant", Proc. SPIE 6868, Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes, 68680D (February 13, 2008); doi:10.1117/12.761423; http://dx.doi.org/10.1117/12.761423


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