We report on the development of a Nd:YVO4 based regenerative amplifier, producing more than 25W of average power at 200kHz, with a pulse length of 10ps at 1064nm. The amplifier is seeded by a low power, mode-locked fiber laser. The amplifier output has been used for the generation of high energy second and third harmonics with up to 80% conversion to 532nm and 40% to 355nm. The average power of the amplifier has been increased further using a single pass linear amplifier to produce 54W at 200kHz.© (2008) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.