Use of adaptive optics to increase nonlinear imaging signal in mouse bone morrow

Yaopeng Zhou; Thomas Bifano; Charles Lin

doi:10.1117/12.769506

8 February 2008 Use of adaptive optics to increase nonlinear imaging signal in mouse bone morrow

Yaopeng Zhou, Thomas Bifano, Charles Lin

Proceedings Volume 6888, MEMS Adaptive Optics II; 688808 (2008) https://doi.org/10.1117/12.769506
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

In a recent effort, researchers from Wellman Center of Photomedicine use fluorescence signal provided by single- or two-photon excitation, second harmonic generation and coherent anti-Stokes Raman spectroscopy (CARS) to illustrate the cell level detail of mouse bone marrow [1]. However, the several non-linear imaging techniques suffered on a common base: signal degradation with deeper light penetration. The fluorescence signal weakening from the mouse skull is caused by the decreased excitation light intensity. With deeper imaging depth, the excitation light suffers tissue scattering, absorption and optical aberration. The last one of the causes spreads the light intensity away from its diffraction limited focal spot. In consequence, less fluorescence light is produced in the enlarged focal volume. In this paper, I will introduce Adaptive Optics (AO), a system for real time optical aberration compensation, to improve the non-linear fluorescence signal in the mouse bone marrow imaging. A parallel stochastic gradient decent algorithm based on Zernike polynomial is employed to control the deformable mirror in real time aberration compensation.

Citation Download Citation

Yaopeng Zhou, Thomas Bifano, and Charles Lin "Use of adaptive optics to increase nonlinear imaging signal in mouse bone morrow", Proc. SPIE 6888, MEMS Adaptive Optics II, 688808 (8 February 2008); https://doi.org/10.1117/12.769506

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available