Development of human granulocyte morphometric model for scanning flow cytometry

Gennady I. Ruban; Svetlana M. Kosmacheva; Natalia V. Goncharova; Valery A. Loiko

doi:10.1117/12.753259

7 August 2007 Development of human granulocyte morphometric model for scanning flow cytometry

Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, Valery A. Loiko

Proceedings Volume 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine; 67341C (2007) https://doi.org/10.1117/12.753259
Event: International Conference on Lasers, Applications, and Technologies '07, 2007, Minsk, Belarus

Abstract

Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.

Citation Download Citation

Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, and Valery A. Loiko "Development of human granulocyte morphometric model for scanning flow cytometry", Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 67341C (7 August 2007); https://doi.org/10.1117/12.753259

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Data modeling

Flow cytometry

Light scattering

Blood

Scattering

Refractive index

Computer simulations

Show All Keywords

Keywords/Phrases

Search In:

Publication Years