Spectroscopic evaluation of time-resolved fluoroimmunoassay

Zhouyi Guo; Zhen Tian; Yali Jia

doi:10.1117/12.546213

8 December 2003 Spectroscopic evaluation of time-resolved fluoroimmunoassay

Zhouyi Guo, Zhen Tian, Yali Jia

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546213
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

The lanthanide trivalence ion and its chelates are used for marking substance in time-resolved fluorescence immunoassay(TRFIA), marking the protein, hormone, antibody, nucleic acid probe or biologic alive cell, to measure the concentration of the analysis substance inside the reaction system with time-resolved fluorometry after the reaction system occurred, and attain the quantitative analysis's purpose. TRFIA has been therefore become a kind of new and more sensitive measure method after radioisotope marking, enzymatic marking, chemiluminescence, electrochemiluminescence, it primarily is decided by the special physics and chemistry characteristic of lanthanide trivalence ion and its chelates. In this paper, the result of spectroscopic evaluation of europium trivalence ion and its chelate, and the principle of time-resolved technology and fluorescence-enhanced technology is reported. At the same time, the experiment shows that excitation wavelength chosen between 336 and 337nm benefits the excitation and the energy transfer of chelate diketone of europium trivalence ion.

Citation Download Citation

Zhouyi Guo, Zhen Tian, and Yali Jia "Spectroscopic evaluation of time-resolved fluoroimmunoassay", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546213

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