Fluorescence correlation spectroscopy based upon two-photon excitation

Yafeng Liu; Tongsheng Chen; Qingming Luo

doi:10.1117/12.546244

4 December 2003 Fluorescence correlation spectroscopy based upon two-photon excitation

Yafeng Liu, Tongsheng Chen, Qingming Luo

Proceedings Volume 5255, Biomolecular Photonics and Multidimensional Microscopy; (2003) https://doi.org/10.1117/12.546244
Event: Biomolecular Photonics and Multidimensional Microscopy, 2003, Wuhan, China

Abstract

Fluorescence correlation spectroscopy (FCS) is a powerful tool for measurement of biological dynamic processes. In this studying, a two-photon excitation fluorescence correlation spectroscopy (TP-FCS) system was set up depending on a part of optical block and detector of the multi-photon excitation fluorescence microscope (MPLFM). The phenomenon "photon-burst" was observed from the TP-FCS system. Meanwhile, the diffusion coefficient of rhodamine B molecule in sucrose aqueous solution was calculated. It was proved that TP-FCS is especially suited for integration into MPEFM accordingly to yield a hybride-technology with the peculiarities of the individual technique and the advantage of mutual synergistic effects. The fusion of both techniques seems to be reasonable and desirable to reduce costs.

Citation Download Citation

Yafeng Liu, Tongsheng Chen, and Qingming Luo "Fluorescence correlation spectroscopy based upon two-photon excitation", Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); https://doi.org/10.1117/12.546244

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