A surface-enhanced Raman scattering (SERS) optical fiber sensor using biconical taper multi-mode fiber (MMF) is proposed. It is fabricated by fused elongating a multi-mode fiber and then coated the silver colloid on its waist. The main advantage of this sensor is that it can increase the SERS active substrate region to make more molecules be excited to produce Raman signal. Silver colloid is selected as the active substrate of SERS. A modified immobilizing process is introduced to increase the thickness of the immobilized layer and reduce the preparation time. Various concentration of R6G is used to detect the performance of the sensor. Strong Raman signal is detected from the waist of the tapered fiber. The detection sensitivity is up to 10-9M.© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.