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Proceedings Article

Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor

[+] Author Affiliations
A. Tyagi, A. Penzkofer

Univ. Regensburg (Germany)

T. Mathes, P. Hegemann

Humboldt Univ. zu Berlin (Germany)

Proc. SPIE 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics, 79931E (January 11, 2011); doi:10.1117/12.879134
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From Conference Volume 7993

  • ICONO 2010: International Conference on Coherent and Nonlinear Optics
  • Kazan, Russian Federation | August 23, 2010

abstract

The wild-type phototropin protein phot from the green alga Chlamydomonas reinhardtii consists of two N-terminal LOV domains LOV1 and LOV2 with flavin mononucleotide (FMN) cofactor and a C-terminal serine-threonine kinase domain. It controls multiple steps in the sexual lifecycle of the alga. Here the LOV1-His domain of phot with modified cofactor is studied. FMN is replaced by roseoflavin monophosphate (8-dimethylamino-8-demethyl-FMN, RoFMN). The modified LOV1 domain is called RoLOV1. The photo-dynamics consequences of the cofactor change are studied. The absorption, emission, and photo-cyclic behaviour of LOV1-His and RoLOV1-His are compared. A spectroscopic characterisation of the cofactors FMN and RoFMN (roseoflavin) is given.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

Topics

Absorption
Citation

A. Tyagi ; A. Penzkofer ; T. Mathes and P. Hegemann
"Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor", Proc. SPIE 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics, 79931E (January 11, 2011); doi:10.1117/12.879134; http://dx.doi.org/10.1117/12.879134


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