Identification of active fluorescence stained bacteria by Raman spectroscopy

Mario Krause; Beatrice Beyer; Christian Pietsch; Benno Radt; Michaela Harz; Petra Rösch; Jürgen Popp

doi:10.1117/12.781321

2 May 2008 Identification of active fluorescence stained bacteria by Raman spectroscopy

Mario Krause, Beatrice Beyer, Christian Pietsch, Benno Radt, Michaela Harz, Petra Rösch, Jürgen Popp

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910E (2008) https://doi.org/10.1117/12.781321
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

Microorganisms can be found everywhere e.g. in food both as useful ingredients or harmful contaminations causing food spoilage. Therefore, a fast and easy to handle analysis method is needed to detect bacteria in different kinds of samples like meat, juice or air to decide if the sample is contaminated by harmful microorganisms. Conventional identification methods in microbiology require always cultivation and therefore are time consuming. In this contribution we present an analysis approach to identify fluorescence stained bacteria on strain level by means of Raman spectroscopy. The stained bacteria are highlighted and can be localized easier against a complex sample environment e.g. in food. The use of Raman spectroscopy in combination with chemometrical methods allows the identification of single bacteria within minutes.

Citation Download Citation

Mario Krause, Beatrice Beyer, Christian Pietsch, Benno Radt, Michaela Harz, Petra Rösch, and Jürgen Popp "Identification of active fluorescence stained bacteria by Raman spectroscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910E (2 May 2008); https://doi.org/10.1117/12.781321

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available