Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy

Sebastian Kosmeier; Björn Kemper; Patrik Langehanenberg; Ilona Bredebusch; Jürgen Schnekenburger; Andreas Bauwens; Gert von Bally

doi:10.1117/12.781125

2 May 2008 Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy

Sebastian Kosmeier, Björn Kemper, Patrik Langehanenberg, Ilona Bredebusch, Jürgen Schnekenburger, Andreas Bauwens, Gert von Bally

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 699110 (2008) https://doi.org/10.1117/12.781125
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

A digital holographic microscopy method for the determination of the integral refractive index of living cells in suspension is presented. Therefore, digital holographic phase contrast images of trypsinized cells in suspension are recorded. Afterwards, the cell radius and the integral cellular refractive index are determined by fitting of a two dimensional model. The applied algorithm requires only information about the refractive index of the suspension medium and the scale of the microscopic imaging system. The fitting algorithm is characterized on simulated phase data and digital holographic phase contrast images of beads. Results obtained from living human pancreas tumor cells demonstrate the applicability of the method for cell characterization.

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Sebastian Kosmeier, Björn Kemper, Patrik Langehanenberg, Ilona Bredebusch, Jürgen Schnekenburger, Andreas Bauwens, and Gert von Bally "Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699110 (2 May 2008); https://doi.org/10.1117/12.781125

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