Laser-induced photoacoustic tomography enhanced with an optical contrast agent

Xueding Wang; Geng Ku; Xueyi Xie; Malgorzata A. Wegiel; Darryl J. Bornhop; George Stoica; Lihong V. Wang

doi:10.1117/12.532434

12 July 2004 Laser-induced photoacoustic tomography enhanced with an optical contrast agent

Xueding Wang, Geng Ku, Xueyi Xie, Malgorzata A. Wegiel, Darryl J. Bornhop, George Stoica, Lihong V. Wang

Proceedings Volume 5320, Photons Plus Ultrasound: Imaging and Sensing; (2004) https://doi.org/10.1117/12.532434
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Optical contrast agents, such as indocyanine dyes, nano-particles and their functional derivatives, have been widely applied to enhance the sensitivity and specificity of optical imaging. However, due to the overwhelming scattering of light in biological tissues, the spatial resolution of traditional optical imaging degrades drastically as the imaging depth increases. For the first time to our knowledge, non-invasive in vivo photoacoustic imaging of an optical contrast agent, distributed in the rat brain, was implemented with near-infrared light. Injection of indocyanine green polyethylene glycol, a contrast agent with a high absorption at the 805-nm wavelength, into the circulatory system of a rat enhanced the absorption contrast between the blood vessels and the background brain tissues. Because near-infrared light can penetrate deep into the brain tissues through the skin and skull, we were able to successfully reconstruct the vascular distribution in the rat brain from the detected photoacoustic signals. The dynamic concentration of this contrast agent in the brain blood after the intravenous injection was also studied. This work proved that the distribution of an exogenous contrast agent in biological tissues can be imaged clearly and accurately by photoacoustic tomography. This new technology has high potential for application in dynamic and molecular medical imaging.

Citation Download Citation

Xueding Wang, Geng Ku, Xueyi Xie, Malgorzata A. Wegiel, Darryl J. Bornhop, George Stoica, and Lihong V. Wang "Laser-induced photoacoustic tomography enhanced with an optical contrast agent", Proc. SPIE 5320, Photons Plus Ultrasound: Imaging and Sensing, (12 July 2004); https://doi.org/10.1117/12.532434

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