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Proceedings Article

Kerr-gated picosecond Raman spectroscopy and Raman photon migration of equine bone tissue with 400-nm excitation

[+] Author Affiliations
Michael D. Morris

Univ. of Michigan (USA)

Allen E. Goodship, Edward R. C. Draper

Royal Veterinary College (United Kingdom)

Pavel Matousek, Michael Towrie, Anthony W. Parker

Rutherford Appleton Lab. (United Kingdom)

Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, 164 (July 1, 2004); doi:10.1117/12.531517
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From Conference Volume 5321

  • Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
  • Anita Mahadevan-Jansen; Michael G. Sowa; Gerwin J. Puppels; Zygmunt Gryczynski; Tuan Vo-Dinh; Joseph R. Lakowicz
  • San Jose, CA | January 24, 2004

abstract

We show that Raman spectroscopy with visible lasers, even in the deep blue is possible with time-gated Raman spectroscopy. A 4 picosec time gate allows efficient fluorescence rejection, up to 1000X, and provides almost background-free Raman spectra with low incident laser power. The technology enables spectroscopy with better than 10X higher scattering efficiency than is possible with the NIR (785 nm and 830 nm) lasers that are conventionally used. Raman photon migration is shown to allow depth penetration. We show for the first time that Kerr-gated Raman spectra of bone tissue with blue laser excitation enables both fluorescence rejection and depth penetration.

© (2004) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Michael D. Morris ; Allen E. Goodship ; Edward R. C. Draper ; Pavel Matousek ; Michael Towrie, et al.
"Kerr-gated picosecond Raman spectroscopy and Raman photon migration of equine bone tissue with 400-nm excitation", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, 164 (July 1, 2004); doi:10.1117/12.531517; http://dx.doi.org/10.1117/12.531517


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