Study of localization of response to fibroblast growth factor-2 in murine calvaria using Raman spectroscopic imaging

Nicole J. Crane; Geng Geng Yu; Michael A. Ignelzi Jr.; Michael D. Morris

doi:10.1117/12.529368

1 July 2004 Study of localization of response to fibroblast growth factor-2 in murine calvaria using Raman spectroscopic imaging

Nicole J. Crane, Geng Geng Yu, Michael A. Ignelzi Jr., Michael D. Morris

Author Affiliations +

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.529368
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Raman spectral imaging provides the means to study spatially localized response to controlled external perturbations to tissue specimens with light microscopy resolution. We discuss use of heparin-acrylic microbeads soaked with the protein fibroblast growth factor-2 (FGF2). Microbeads containing FGF2 are placed in murine calvarial tissue and stimulate abnormally rapid mineralization. The tissue response simulated the effects of craniosynostosis, a birth defect occurring in 1 in 2400 live births. We describe Raman imaging measurements of the spatial distribution of apatitic mineral and matrix (predominantly type I collagen) from normal murine calvarial tissue and murine calvarial tissue modeling craniosynostosis. We also discuss spectroscopic evaluation of the state of the mineral induced by the FGF2 beads.

Citation Download Citation

Nicole J. Crane, Geng Geng Yu, Michael A. Ignelzi Jr., and Michael D. Morris "Study of localization of response to fibroblast growth factor-2 in murine calvaria using Raman spectroscopic imaging", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.529368

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