Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Shot-noise limited detection sensitivity in multiplex CARS microscopy

[+] Author Affiliations
Michiel Mueller, George W. H. Wurpel

Univ. of Amsterdam (Netherlands)

Juleon M. Schins

Delft Univ. of Technology (Netherlands)

Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, 195 (June 21, 2004); doi:10.1117/12.527924
Text Size: A A A
From Conference Volume 5323

  • Multiphoton Microscopy in the Biomedical Sciences IV
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 24, 2004

abstract

In multiplex CARS microscopy the generated anti-Stokes signal is generated and detected simultaneously over a significant part of the vibrational spectrum. The signal-to-noise ratio of the thus detected spectra is limited only by shot-noise. This principle is demonstrated using a dilution series of 2-propanol in water. It is derived theoretically and shown experimentally that for low solute concentrations - in contrast to methods that suppress the non-resonant background - the CARS signal strength from a particular vibrational mode depends linearly on its concentration. Furthermore, excellent agreement is shown between the experimental data and fits to the theory. It is shown that this approach permits rapid (20 ms acquisition) detection of a single lipid mono-layer, with sufficient signal-to-noise to determine the order parameter for the acyl chain packing. Also it is demonstrated that this detection scheme provides an absolute measure of the solute concentration.

© (2004) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Michiel Mueller ; Juleon M. Schins and George W. H. Wurpel
"Shot-noise limited detection sensitivity in multiplex CARS microscopy", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, 195 (June 21, 2004); doi:10.1117/12.527924; http://dx.doi.org/10.1117/12.527924


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.