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Proceedings Article

Multichannel multiphoton imaging of metal oxides nanoparticles in biological system

[+] Author Affiliations
Yuangang Zheng, Gary Holtom, Steven D. Colson

Pacific Northwest National Lab. (USA)

Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, 390 (June 21, 2004); doi:10.1117/12.528337
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From Conference Volume 5323

  • Multiphoton Microscopy in the Biomedical Sciences IV
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 24, 2004

abstract

Near-IR ultrafast pulse laser and confocal microscope are combined to create a multiphoton multichannel non-linear imaging technique, which allows in situ 3-D characterization of nonfluorescent nanoparticles in biological systems. We observed intense CARS signals generated from various metal oxides due to their high third-order nonlinear susceptibilities (Chi(3)), which do not depend on the vibrational resonance but on the electronic resonance. We show that fine and ultrafine particles of metal oxides in alveolar macrophage cells may be imaged in vitro using CARS and multiphoton fluorescence microscopy with highest optical resolution for extended periods without photobleaching effects. The advantage of the epi-detection over the forward detection for imaging sub-micron particles has been investigated.

© (2004) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Yuangang Zheng ; Gary Holtom and Steven D. Colson
"Multichannel multiphoton imaging of metal oxides nanoparticles in biological system", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, 390 (June 21, 2004); doi:10.1117/12.528337; http://dx.doi.org/10.1117/12.528337


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