The use of multi-modal optical diagnostic approach has a great potential in medicine and biology allowing significantly expand the capabilities of vascular diagnostics. Multi-modal approach provides synchronic in vivo images of blood and lymph vessels. In current study the Dynamic Light Scattering approach has been used for rendering blood vessels structure and monitoring blood micro-flows in the mouse ear in vivo with the higher spatial resolution, whereas conventional Fluorescence Intravital Microscopy provides simultaneous image of lymphatic vessels. The results clearly demonstrate that combined application of Dynamic Light Scattering and conventional Fluorescence Intravital Microscopy approaches provides synchronic in vivo images of blood and lymph vessels as well as blood micro-flows with a high contrast and specificity.© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.