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Proceedings Article

Extracting the ridge set as a graph for quantification of actin filament images obtained by confocal laser scanning microscopy

[+] Author Affiliations
Harald Birkholz

Univ. of Rostock (Germany)

Proc. SPIE 8000, Tenth International Conference on Quality Control by Artificial Vision, 800006 (July 12, 2011); doi:10.1117/12.895269
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From Conference Volume 8000

  • Tenth International Conference on Quality Control by Artificial Vision
  • Jean-Charles Pinoli; Johan Debayle; Yann Gavet; Frédéric Gruy; Claude Lambert
  • Saint-Etienne, France | June 28, 2011

abstract

The progress in image acquisition technique provides life sciences with an abundance of data. Image analysis facilitates the assessment. The actin cytoskeleton plays a crucial role in understanding the behavior of osteoblastic cells on biomaterials. It can be visualized by confocal laser scanning microscopy. In these images it appears as a dense network of bright ridges which is so far only qualitatively assessed. For quantification there is a need for ridge detection techniques which provide a geometrical description of this graph-like feature. The state of the art methods do not cope with the systematical degradation of the brightness information. This paper presents the key part of a ridge tracking technique, which makes more efficient use of context information. Two random models confirm the accuracy and highlight the area for further improvement of the method.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Harald Birkholz
"Extracting the ridge set as a graph for quantification of actin filament images obtained by confocal laser scanning microscopy", Proc. SPIE 8000, Tenth International Conference on Quality Control by Artificial Vision, 800006 (July 12, 2011); doi:10.1117/12.895269; http://dx.doi.org/10.1117/12.895269


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