Holographic interferometric microscopy systems for the application on biological samples

Bjorn Kemper; Daniel Carl; Sabine Knoche; Rainer Thien; Gert von Bally

doi:10.1117/12.543996

10 September 2004 Holographic interferometric microscopy systems for the application on biological samples

Bjorn Kemper, Daniel Carl, Sabine Knoche, Rainer Thien, Gert von Bally

Proceedings Volume 5457, Optical Metrology in Production Engineering; (2004) https://doi.org/10.1117/12.543996
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Holographic interferometric metrology allows a fast, non destructive and quantitative high resolution full field detection of optical path length changes. Furthermore, by utilization of modern CCD sensor technology and digital image process-ing algorithms an on-line application of these methods even on biological specimens is possible. In combination with a microscopic resolution this offers new possibilities for the detection of variations of shape, micro movements or refractive index changes e. g. for the marker free analysis of cellular samples. Three holographic interferometric systems for microscopy applications based on digital holography, (speckle) interferometry and photorefractive crystals as holographic recording medium are introduced. Results of investigations on test charts and biological samples to characterize and optimize the lateral resolution as well as the resolution of the detected phase difference changes are presented and discussed. Finally, the applicability of the developed measurement techniques on living cells is demonstrated.

Citation Download Citation

Bjorn Kemper, Daniel Carl, Sabine Knoche, Rainer Thien, and Gert von Bally "Holographic interferometric microscopy systems for the application on biological samples", Proc. SPIE 5457, Optical Metrology in Production Engineering, (10 September 2004); https://doi.org/10.1117/12.543996

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