Development of a biosensor based on a prism coupler method

Stephen Couvignou; Luc Trudel; Roger A. Lessard; Louise Brisson

doi:10.1117/12.543668

1 September 2004 Development of a biosensor based on a prism coupler method

Stephen Couvignou, Luc Trudel, Roger A. Lessard, Louise Brisson

Proceedings Volume 5459, Optical Sensing; (2004) https://doi.org/10.1117/12.543668
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Agar is a complex mixture of polysaccharides. It is widely used in microbiology as a jellifying agent to obtain solid culture medium that can then support the growth of bacteria, yeasts and other microorganisms. Although this substrate has a daily use in biology and microbiology, its physico-chemical properties are still not well known. Consequently, a characterisation of a solid bacteriological medium infected or not by Escherichia coli type bacteria can be realized on thin films obtained by spin coating. The present study using a prism coupler method gives a sensitive tool to detect photo-physical change in the medium. This method could allow us to detect the presence of bacteria. Results obtained are reproducible on different samples and might be qualitatively compared with conventional imagery. This method should enable a rapid detection of the growing of bacteria on the studied medium.

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Stephen Couvignou, Luc Trudel, Roger A. Lessard, and Louise Brisson "Development of a biosensor based on a prism coupler method", Proc. SPIE 5459, Optical Sensing, (1 September 2004); https://doi.org/10.1117/12.543668

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