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Proceedings Article

Dynamic correction of aberrations in microscopic imaging systems using an artificial point source

[+] Author Affiliations
Marcus Reicherter, Witold Grski, Tobias Haist, Wolfgang Osten

Univ. Stuttgart (Germany)

Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, 68 (September 10, 2004); doi:10.1117/12.544115
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From Conference Volume 5462

  • Biophotonics Micro- and Nano-Imaging
  • Dario Anselmetti
  • Strasbourg, France | April 26, 2004

abstract

In biological micromanipulation image aberrations are introduced not only by the optical system, but also by the immersion liquid. Whereas optical system aberrations are constant and it is relatively easy to measure and correct for them, the immersion caused aberrations are variable in time and space. In this paper a method using a spherical microparticle as an artificial point source for aberration control is presented. The particle is positioned by optical tweezers at the location of the biological sample. In the experiment holographic tweezers are used. They are based on computer generated holograms, written into spatial light modulators, which create light traps for the microparticle in the object plane. The light traps can be moved without any mechanically moving parts, just by changing the hologram. The particle strongly focuses the light, therefore an artificial point source in the object space is created. The illumination light is filtered, so that only the signal corresponding to a spherical wave is analyzed by the wavefront detection system. The information about the wavefront distortion is used to dynamically correct for it. This can be done by using spatial light modulators. The method is suitable for biophotonic imaging systems, where refractive index variations in the sample plane are significant. The integration with holographic tweezers is advantageous since it offers flexibility in positioning and imaging the particles.

© (2004) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Marcus Reicherter ; Witold Grski ; Tobias Haist and Wolfgang Osten
"Dynamic correction of aberrations in microscopic imaging systems using an artificial point source", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, 68 (September 10, 2004); doi:10.1117/12.544115; http://dx.doi.org/10.1117/12.544115


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