Cell-based bioassays in microfluidic systems

Laura J. Itle; Jeanna C. Zguris; Michael V. Pishko

doi:10.1117/12.569094

7 December 2004 Cell-based bioassays in microfluidic systems

Laura J. Itle, Jeanna C. Zguris, Michael V. Pishko

Proceedings Volume 5588, Smart Medical and Biomedical Sensor Technology II; (2004) https://doi.org/10.1117/12.569094
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

The development of cell-based bioassays for high throughput drug screening or the sensing of biotoxins is contingent on the development of whole cell sensors for specific changes in intracellular conditions and the integration of those systems into sample delivery devices. Here we show the feasibility of using a 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate, a fluorescent dye capable of responding to changes in intracellular pH, as a detection method for the bacterial endotoxin, lipopolysaccharide. We used photolithography to entrap cells with this dye within poly(ethylene) glyocol diacrylate hydrogels in microfluidic channels. After 18 hours of exposure to lipopolysaccharide, we were able to see visible changes in the fluorescent pattern. This work shows the feasibility of using whole cell based biosensors within microfluidic networks to detect cellular changes in response to exogenous agents.

Citation Download Citation

Laura J. Itle, Jeanna C. Zguris, and Michael V. Pishko "Cell-based bioassays in microfluidic systems", Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); https://doi.org/10.1117/12.569094

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