Comparative study of the photodynamic effect in tumor and nontumor animal cell lines

Elena V. Stoykova; R. Alexandrova; Stanislav Shurulinkov; O. Sabotinov; Georgi Minchev

doi:10.1117/12.584432

10 September 2004 Comparative study of the photodynamic effect in tumor and nontumor animal cell lines

Elena V. Stoykova, R. Alexandrova, Stanislav Shurulinkov, O. Sabotinov, Georgi Minchev

Proceedings Volume 5610, Laser Florence 2003: A Window on the Laser Medicine World; (2004) https://doi.org/10.1117/12.584432
Event: Laser Florence 2003: A Window on the Laser Medicine World, 2003, Florence, Italy

Abstract

In this study we evaluate the cytotoxicity of two photosensitisers with absorption peaks in the green and red part of the spectrum on animal cell lines. The cytotoxicity assessment was performed for a tumor cell line LSCC-SF-Mc29, obtained from a transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29, a tumor line LSR-SF-SR, obtained from a transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and for normal mouse and bovine cell lines. Up to now the effect of the photodynamic therapy on virus-induced cancers has not been clarified. The cells were treated with 5,10,15,20 - tetra (4-sulfophenyl) porphyrin with main absorption peak at 519 nm and a dye activated with a red light. The cells were seeded in 96-well plates at 2 x 10⁴ cells/well. The cells were exposed to irradiation from a pulsed CuBr vapor laser at 510.6 nm and 578.2 nm and exposure rate 50 mW/cm², from an Ar-ion laser at 514 nm and 1 mW/cm² and to 655 nm-irradiation from a semiconductor laser at 10 mW/cm². The biological activity of the tested compounds was measured by the neutral red uptake cytotoxicity test. The light dose-response curves and light exposures that ensure 50% drop in the treated cells viability in comparison with the cells grown in non-modified medium were obtained for each cell line. The cytotoxic effect of both photosensitisers is most distinguished for the tumor line LSCC-SF-Mc29. The 2-4 times higher viability of the normal cell lines in comparison with the tumor lines is established. The bovine cell lines are more vulnerable than the mouse lines.

Citation Download Citation

Elena V. Stoykova, R. Alexandrova, Stanislav Shurulinkov, O. Sabotinov, and Georgi Minchev "Comparative study of the photodynamic effect in tumor and nontumor animal cell lines", Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); https://doi.org/10.1117/12.584432

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