Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe

Zhaohui Hu; Jia Wang; Jinwen Liang

doi:10.1117/12.575668

18 January 2005 Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe

Zhaohui Hu, Jia Wang, Jinwen Liang

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.575668
Event: Photonics Asia, 2004, Beijing, China

Abstract

In conventional optical tweezers system a high numerical aperture (NA) objective is employed both to image and to generate a gradient force toward the focus on sample particles, so the system is complex and expensive especially for the multi-optical-tweezers system. We built a novel simple optical trapping system based on a lensed optical fiber probe. This new method offers several other advantages over the conventional optical tweezers. The trapping system we built includes a laser coupling unit, a multi-dimensional probe manipulating unit, a sample nano-positioning unit, and a microscopy imaging unit. Based on the system, a yeast cell is trapped and manipulated on the chamber bottom by the lensed fiber probe, and the optical trapping forces acting on the yeast cell as a function of the offset are measured and discussed in different directions by the static method and the dynamic one respectively with various powers. The results by the two measurement methods coincide with each other, and the detail experimental procedure and the data processing of the two methods are introduced in this paper.

Citation Download Citation

Zhaohui Hu, Jia Wang, and Jinwen Liang "Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.575668

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