Development of an electro-responsive platform for the controlled transfection of mammalian cells

Andrew L. Hook; Helmut W. Thissen; Jason P. Hayes; Nicolas H. Voelcker

doi:10.1117/12.606789

16 February 2005 Development of an electro-responsive platform for the controlled transfection of mammalian cells

Andrew L. Hook, Helmut W. Thissen, Jason P. Hayes, Nicolas H. Voelcker

Proceedings Volume 5651, Biomedical Applications of Micro- and Nanoengineering II; (2005) https://doi.org/10.1117/12.606789
Event: Smart Materials, Nano-, and Micro-Smart Systems, 2004, Sydney, Australia

Abstract

The recent development of living microarrays as novel tools for the analysis of gene expression in an in-situ environment promises to unravel gene function within living organisms. In order to significantly enhance microarray performance, we are working towards electro-responsive DNA transfection chips. This study focuses on the control of DNA adsorption and desorption by appropriate surface modification of highly doped p++ silicon. Silicon was modified by plasma polymerisation of allylamine (ALAPP), a non-toxic surface that sustains cell growth. Subsequent high surface density grafting of poly(ethylene oxide) formed a layer resistant to biomolecule adsorption and cell attachment. Spatially controlled excimer laser ablation of the surface produced micron resolution patterns of re-exposed plasma polymer whilst the rest of the surface remained non-fouling. We observed electro-stimulated preferential adsorption of DNA to the ALAPP surface and subsequent desorption by the application of a negative bias. Cell culture experiments with HEK 293 cells demonstrated efficient and controlled transfection of cells using the expression of green fluorescent protein as a reporter. Thus, these chemically patterned surfaces are promising platforms for use as living microarrays.

Citation Download Citation

Andrew L. Hook, Helmut W. Thissen, Jason P. Hayes, and Nicolas H. Voelcker "Development of an electro-responsive platform for the controlled transfection of mammalian cells", Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); https://doi.org/10.1117/12.606789

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