Effect of Light Emitting Diode (LED) therapy on the survival of photoreceptors following Argon laser injury

Cheryl Dawn DiCarlo D.V.M.; Jeremiah Brown M.D.; Henry D. Hacker M.D.; Steven T. Schuschereba; Rachael D. Cheramie; James M. Sankovich; Harry Zwick; David J. Lund; Bruce E. Stuck

doi:10.1117/12.593670

18 April 2005 Effect of Light Emitting Diode (LED) therapy on the survival of photoreceptors following Argon laser injury

Cheryl Dawn DiCarlo D.V.M., Jeremiah Brown M.D., Henry D. Hacker M.D., Steven T. Schuschereba, Rachael D. Cheramie, James M. Sankovich, Harry Zwick, David J. Lund, Bruce E. Stuck

Author Affiliations +

Proceedings Volume 5688, Ophthalmic Technologies XV; (2005) https://doi.org/10.1117/12.593670
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Due to the increasing number of optic systems that military personnel are exposed, the development of countermeasures for laser eye injury is of significant concern. Recent reports in the literature suggest some benefit form the use of Light Emitting Diode (LED) therapy on the retina that received a toxic insult. The purpose of this study was to compare retinal cell survival and multifocal electroretinography (mfERG) in a laser retinal injury model following treatment with LED photoillumination. Control and LED array (670 nm) illuminated cynomolgus monkeys received macular Argon laser lesions (514 nm, 130 mW, 100 ms). LED array exposure was accomplished for 4 days for a total dose of 4 J/cm² per day. Baseline and post-laser exposure mfERGs were performed on most of the subjects. Ocular tissues were collected from four animals at Day 4 poast laser exposure and from two animals at 4 months post laser exposure. The tissues were processed for plastic embedding. Retinal cell counts were performed on the lesion sections. Analysis of Variance (ANOVA) results yielded no significant difference in the sparing of photoreceptors, inner nuclear and ganglion cells between the control and LED illuminated subjects. Although pathology showed no significant support for diode therapy, our early mfERG observations previously reported suggested a more rapid functional recovery. Since there is still no uniform therapy for laser retinal injury, research is continuing to determine novel therapies that may provide retinal cell sparing and functional retinal return.

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Cheryl Dawn DiCarlo D.V.M., Jeremiah Brown M.D., Henry D. Hacker M.D., Steven T. Schuschereba, Rachael D. Cheramie, James M. Sankovich, Harry Zwick, David J. Lund, and Bruce E. Stuck "Effect of Light Emitting Diode (LED) therapy on the survival of photoreceptors following Argon laser injury", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); https://doi.org/10.1117/12.593670

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