Instrumentation considerations in spectral imaging for tissue demarcation: comparing three methods of spectral resolution

Steven Charles Gebhart; David L. Stokes; Tuan Vo-Dinh; Anita Mahadevan-Jansen

doi:10.1117/12.611351

23 March 2005 Instrumentation considerations in spectral imaging for tissue demarcation: comparing three methods of spectral resolution

Steven Charles Gebhart, David L. Stokes, Tuan Vo-Dinh, Anita Mahadevan-Jansen

Author Affiliations +

Proceedings Volume 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III; (2005) https://doi.org/10.1117/12.611351
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Multiple methodologies exist to implement spectral imaging for tissue demarcation and disease diagnosis. In this paper, benchtop acousto-optic tunable filter (AOTF), liquid-crystal tunable filter (LCTF) and Fourier interferometric spectral imaging systems were quantitatively compared in terms of imaging speed of soft tissue autofluorescence. Optical throughput, image signal-to-noise ratio (SNR), and collagen autofluorescence imaging in chicken breast were assessed. Within this comparison, the Fourier system possessed the largest optical throughput (~50%) relative to the tunable-filter imaging systems; however, its throughput advantage failed to correlate to improved image SNR over the LCTF system. Further, while the autofluorescence imaging capability of the Fourier system exceeded that of the LCTF system for comparable total image integration times, the LCTF is capable of producing equivalent autofluorescence SNR with superior SNR when interrogations at only a few wavelengths are required and the random access filter tuning of the LCTF can be exploited. Therefore, the simple, rugged design and random-access filter-tuning capability of LCTF-based spectral imaging makes it best-suited for clinical development of soft tissue autofluorescence imaging.

Citation Download Citation

Steven Charles Gebhart, David L. Stokes, Tuan Vo-Dinh, and Anita Mahadevan-Jansen "Instrumentation considerations in spectral imaging for tissue demarcation: comparing three methods of spectral resolution", Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); https://doi.org/10.1117/12.611351

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available