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Proceedings Article

Refractive index determination from multiphoton and confocal spherical aberration microscopy

[+] Author Affiliations
Peter T. Fwu, Wen Lo, Chen-Yuan Dong

National Taiwan Univ. (Taiwan)

Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, 70 (April 11, 2005); doi:10.1117/12.587255
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From Conference Volume 5700

  • Multiphoton Microscopy in the Biomedical Sciences V
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 22, 2005

abstract

Refractive index mismatch induced spherical aberration (RIMISA) is a ubiquitous problem for in-depth optical biological imaging and it is an effect most researchers like to avoid. Associated with RIMISA is a broadening of the point-spread-function (PSF) which leads to a degradation of image resolution and the loss of specimen signal. It is these features that render RIMISA to be useful for one important application in biological application, the determination of sample refractive index. We will show how RIMISA under multiphoton and confocal microscopy can be used to determine the refractive indices of uniformly luminescent specimens and a comparison will be made between the multiphoton and confocal approaches.

© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Peter T. Fwu ; Wen Lo and Chen-Yuan Dong
"Refractive index determination from multiphoton and confocal spherical aberration microscopy", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, 70 (April 11, 2005); doi:10.1117/12.587255; http://dx.doi.org/10.1117/12.587255


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