Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

A novel concept for a mid-field microscope

[+] Author Affiliations
Margreet W. Docter, I. T. Young, Vladimir G. Kutchoukov, Andre Bossche, Paul F. Alkemade, Yuval Garini

Technische Univ. Delft (Netherlands)

Proc. SPIE 5703, Plasmonics in Biology and Medicine II, 118 (April 11, 2005); doi:10.1117/12.588216
Text Size: A A A
From Conference Volume 5703

  • Plasmonics in Biology and Medicine II
  • Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski
  • San Jose, CA | January 22, 2005

abstract

When light passes through a hole smaller than the wavelength of the light, the transmission is very low and the light is diffracted. This however changes if holes are arranged in a periodic array on metal. In that case the light couples to surface plasmons; this results in enhanced transmission, spectral selection and a small angular diffraction. We develop a novel microscopic method based on a periodic hole-array, which will be used as a multiple-apertures near-field source for illuminating a biological sample while the light is collected in far-field. The measurement speed is high, due to the use of an array instead of a single source. The main advantage of this microscope originates from the low diffraction of light through a relatively thick sample with enhanced transmission. It results in the ability to measure the samples interior and 3D reconstruction can be made by semi-confocal techniques. This overcomes the major limitation of near-field methods for which only a shallow layer of the surface (~20 nm) is detectable. For our measurements we use glass coated devices. The holes are processed with a focused ion beam. The photon-plasmon coupling process is characterized as a function of the wavelength. Our experiments aim on gaining a better understanding of the transmission process. We tested the dependence of the transmitted spectrum on angle of incidence was tested as well as far-field spectral imaging measurements of the transmission in both Koehler and collimated light illumination. The results as well as the description of the microscope that we are constructing are presented.

© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Margreet W. Docter ; I. T. Young ; Vladimir G. Kutchoukov ; Andre Bossche ; Paul F. Alkemade, et al.
"A novel concept for a mid-field microscope", Proc. SPIE 5703, Plasmonics in Biology and Medicine II, 118 (April 11, 2005); doi:10.1117/12.588216; http://dx.doi.org/10.1117/12.588216


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.