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Proceedings Article

Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters

[+] Author Affiliations
Mounir Bouhifd, Maurice P. Whelan

European Commission/Joint Research Ctr. (Italy)

Marc Aprahamian

INSERM (France)

Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, 185 (July 14, 2005); doi:10.1117/12.605088
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From Conference Volume 5826

  • Opto-Ireland 2005: Optical Sensing and Spectroscopy
  • Hugh J. Byrne; Elfed Lewis; Brian D. MacCraith; Enda McGlynn; James A. McLaughlin; Gerard D. O'Sullivan; Alan G. Ryder; James E. Walsh
  • Dublin, Ireland | April 04, 2005

abstract

A novel prototype instrument for mutli-spectral imaging applications is described. The device is based on an acousto-optic tuneable filter (AOTF) that can be easily attached to many standard imaging systems (e.g. endoscope or fluorescence microscope). The instrument developed offers significant advantages over typical fixed-filter based systems in terms of flexibility, performance and diagnostic potential. The selected AOTF was designed to have a large acceptance aperture suitable for imaging applications. Any filtering centre-wavelength in the visible range (450 to 700nm) can be rapidly selected by either random access or by continuous tuning thus providing a versatile performance. The prototype instrument has been demonstrated for in-vivo applications where it was attached to the eyepiece of a commercial endoscope allowing simultaneous white light and fluorescence endoscopy. Autofluorescence of endogenous protoporphyrin IX (PpIX), a biomarker of diseased tissues undergoing an inflammatory response, was mapped in vivo on a rat model. The AOTF device was also coupled to the viewing port of a commercial fluorescence microscope thus realising a powerful fluorescence imaging spectrometer capable of detecting and mapping fluorescent biomolecules in vitro.

© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Mounir Bouhifd ; Maurice P. Whelan and Marc Aprahamian
"Fluorescence imaging spectroscopy utilising acousto-optic tuneable filters", Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, 185 (July 14, 2005); doi:10.1117/12.605088; http://dx.doi.org/10.1117/12.605088


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