Effect of scanning rate on the image contrast in confocal microscopy for biological application

Byung Seon Chun; Tae Joong Kim; Incheon Song; Dae-Gab Gweon; Jaebum Choo; Chil Hwan Oh

doi:10.1117/12.615825

1 August 2005 Effect of scanning rate on the image contrast in confocal microscopy for biological application

Byung Seon Chun, Tae Joong Kim, Incheon Song, Dae-Gab Gweon, Jaebum Choo, Chil Hwan Oh

Proceedings Volume 5873, Optical Scanning 2005; (2005) https://doi.org/10.1117/12.615825
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

In this research, the method how to estimate the image quality for different scanning rate is suggested and experimentally shown with the laboratory-built confocal laser scanning microscope. The confocal microscope is designed for in vivo reflectance imaging of a biological tissue, which uses the refractive index mismatch at the boundaries of a tissue to generate an image without any additional staining process. The two-dimensional scanning mechanism is built up with a polygonal mirror and a galvanometric mirror that can be controlled to operate at a specific speed. To examine the effect of scanning rate on the image contrast, confocal scanning images of a biological specimen are acquired with various scanning rate while the other conditions are kept same. The contrast of confocal microscopic image is transformed into the numeric expression to describe the relation between image contrast and scanning rate quantitatively. Results suggest some useful methodology of how to determine the allowable maximum scanning rate for the specific application of confocal microscopy.

Citation Download Citation

Byung Seon Chun, Tae Joong Kim, Incheon Song, Dae-Gab Gweon, Jaebum Choo, and Chil Hwan Oh "Effect of scanning rate on the image contrast in confocal microscopy for biological application", Proc. SPIE 5873, Optical Scanning 2005, (1 August 2005); https://doi.org/10.1117/12.615825

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available