Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization

Feng-Ming Hsiu; Shean-Jen Chen; Chien-Hung Tsai; Chia-Yuan Tsou; Y.-D. Su; G.-Y. Lin; K.-T. Huang; Jin-Jung Chyou; Wei-Chih Ku; S.-K. Chiu; C.-M. Tzeng

doi:10.1117/12.450922

25 September 2002 Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization

Feng-Ming Hsiu, Shean-Jen Chen, Chien-Hung Tsai, Chia-Yuan Tsou, Y.-D. Su, G.-Y. Lin, K.-T. Huang, Jin-Jung Chyou, Wei-Chih Ku, S.-K. Chiu, C.-M. Tzeng

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Proceedings Volume 4819, Polarization Measurement, Analysis, and Applications V; (2002) https://doi.org/10.1117/12.450922
Event: International Symposium on Optical Science and Technology, 2002, Seattle, WA, United States

Abstract

Surface plasmon resonance (SPR) imaging system is presented as a novel technique based on modified Mach-Zehnder phase-shifting interferometry (PSI) for biomolecular interaction analysis (BIA), which measures the spatial phase variation of a resonantly reflected light in biomolecular interaction. In this technique, the micro-array SPR biosensors with over a thousand probe NDA spots can be detected simultaneously. Owing to the feasible and swift measurements, the micro-array SPR biosensors can be extensively applied to the nonspecific adsorption of protein, the membrane/protein interactions, and DNA hybridization. The detection sensitivity of the SPR PSI imaging system is improved to about 1 pg/mm² for each spot over the conventional SPR imaging systems. The SPR PSI imaging system and its SPR sensors have been successfully used to observe slightly index change in consequence of argon gas flow through the nitrogen in real time, with high sensitivity, and at high-throughout screening rates.

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Feng-Ming Hsiu, Shean-Jen Chen, Chien-Hung Tsai, Chia-Yuan Tsou, Y.-D. Su, G.-Y. Lin, K.-T. Huang, Jin-Jung Chyou, Wei-Chih Ku, S.-K. Chiu, and C.-M. Tzeng "Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization", Proc. SPIE 4819, Polarization Measurement, Analysis, and Applications V, (25 September 2002); https://doi.org/10.1117/12.450922

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