In-line digital holography conciles the applicative interest of a simple optical set-up with the speed, low cost and potential of digital reconstruction. We address the twin-image problem that arises in holography due to the lack of phase information in intensity measurements. This problem is of great importance in in-line holography where spatial elimination of the twin-image cannot be carried out as in off-axis holography. Applications in digital holography of particle fields greatly depend on its suppression to reach greater particle concentrations, keeping a sufficient signal to noise ratio in reconstructed images. We describe in this paper methods to improve numerically the reconstructed images by twin-image reduction.© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.