Measurement of singlet oxygen luminescence from AML5 cells sensitized with ALA-induced PpIX in suspension during photodynamic therapy and correlation with cell viability after treatment

Mark J. Niedre; Michael S. Patterson; Natalie Boruvka; Brian C. Wilson

doi:10.1117/12.469336

6 June 2002 Measurement of singlet oxygen luminescence from AML5 cells sensitized with ALA-induced PpIX in suspension during photodynamic therapy and correlation with cell viability after treatment

Mark J. Niedre, Michael S. Patterson, Natalie Boruvka, Brian C. Wilson

Author Affiliations +

Proceedings Volume 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI; (2002) https://doi.org/10.1117/12.469336
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Photodynamic therapy (PDT) treatments were performed on suspensions of AML5 cells incubated with ALA-induced PpIX with either 15 mW/cm², 45 mW/cm² or 90 mW/cm² pulsed 523 nm light. Singlet oxygen (¹O₂) luminescence measurements were carried out using a detection system including a near-infrared (NIR) sensitive photomultiplier tube (PMT). Cell viability was determined at several time points during treatments using the propidium iodide (PI) flow cytometry assay. Cumulative ¹O₂ measurements were compared to cell viability for all experiments. Preliminary results show that average cell viability increased with increasing treatment irradiance. Cell viability was found to correlate well with absolute cumulative ¹O₂ luminescence measurements during treatment.

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Mark J. Niedre, Michael S. Patterson, Natalie Boruvka, and Brian C. Wilson "Measurement of singlet oxygen luminescence from AML5 cells sensitized with ALA-induced PpIX in suspension during photodynamic therapy and correlation with cell viability after treatment", Proc. SPIE 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI, (6 June 2002); https://doi.org/10.1117/12.469336

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