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Proceedings Article

Characterization of cervical normal and abnormal tissues by synchronous luminescence spectroscopy

[+] Author Affiliations
Nammalver Vengadesan, Srinivasan Hemamalini, Jeyasingh Ebenezar, Prakasa R. Aruna, Singaravelu Ganesan

Anna Univ. (India)

Thalaimuthu Anbupalam

Univ. of Madras (India)

Kulandhaivel Muthvelu

Government Arignar Anna Memoral Cancer Hospital (India)

D. Koteeswaran

Government Arignar Anna Memorial Cancer Hospital (India)

Proc. SPIE 4613, Optical Biopsy IV, 13 (May 7, 2002); doi:10.1117/12.465247
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From Conference Volume 4613

  • Optical Biopsy IV
  • Robert R. Alfano
  • San Jose, CA | January 19, 2002

abstract

Fluorescence spectroscopy has emerged as a promising modality in the discrimination of normal from pathologically diseased cells and tissues. As tissues are highly heterogeneous with many native fluorophores, emission spectra at one or more excitation wavelengths or excitation spectra corresponding to one or more emission wavelengths are used for diagnostic purpose. This could be overcome to great extent by applying synchronous luminescence spectroscopy. In the present study, synchronous luminescence fluorescence spectra of normal, pre malignant and malignant cervical tissues are measured by scanning both excitation and emission monochromator simultaneously with a wavelength difference of 20nm. The synchronous luminescence spectra of normal, pre-malignant and malignant cervical tissues shows the distinct peaks around 300, 350, 525nm with broad peak around 460nm and this may be due to tryptophan, collagen and flavin respectively. The broad band around 460nm may be due to the presence of pyridoxal phosphate, carotenes and lipopigments. Spectral data are also evaluated by both empirical and statistical analysis. Among the various analysis partial least square analysis provides better accuracy than other analysis in the discrimination of normal from abnormal tissues.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Nammalver Vengadesan ; Thalaimuthu Anbupalam ; Srinivasan Hemamalini ; Jeyasingh Ebenezar ; Kulandhaivel Muthvelu, et al.
"Characterization of cervical normal and abnormal tissues by synchronous luminescence spectroscopy", Proc. SPIE 4613, Optical Biopsy IV, 13 (May 7, 2002); doi:10.1117/12.465247; http://dx.doi.org/10.1117/12.465247


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