Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy

[+] Author Affiliations
Vadim Backman, Irving Itzkan

Northwestern Univ. (USA)

Rajan S. Gurjar, Maxim Kalashnikov, Adam Wax, Irene Georgakoudi, Markus G. Mueller, Charles W. Boone, Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology (USA)

Lev T. Perelman

Harvard Medical School (USA)

Venkatesh Gopal

Massachusetts Institute of Technology (India)

Kamran Badizadegan

Children's Hospital/Harvard Medical School (USA)

Proc. SPIE 4613, Optical Biopsy IV, 101 (May 7, 2002); doi:10.1117/12.465234
Text Size: A A A
From Conference Volume 4613

  • Optical Biopsy IV
  • Robert R. Alfano
  • San Jose, CA | January 19, 2002

abstract

Light scattering spectroscopy (LSS) is a promising optical technique developed for quantitative characterization of tissue morphology as well as in vivo detection and diagnosis of disease such as early cancer. LSS employs a wavelength dependent component of light scattered by epithelial cells and other tissues to obtain information about subcellular structure. We present two novel modalities of LSS, LSS imaging and scattering angle sensitive LSS (a/LSS). LSS imaging provides quantitative information about the epithelial cell nuclei, such as nuclear size, degree of pleomorphism, hyperchromasia, and amount of chromatin. It allows mapping these histological properties over wide areas of epithelial lining. We show that LSS imaging can be used to detect precancerous lesions in optically accessible organs. Using a/LSS, which enables characterization of tissue components with sizes smaller than the wavelength of light, we show that the number of subcellular components with the sizes between 30 nm and few microns scales with the size according to an inverse power-law. We show that the size distribution exponent is an important parameter characterizing tissue organization, for example the balance between stochasticity and order, and has a potential to be applicable for early cancer diagnosis and characterization.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Vadim Backman ; Rajan S. Gurjar ; Lev T. Perelman ; Venkatesh Gopal ; Maxim Kalashnikov, et al.
"Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy", Proc. SPIE 4613, Optical Biopsy IV, 101 (May 7, 2002); doi:10.1117/12.465234; http://dx.doi.org/10.1117/12.465234


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.