Functional imaging of photosensitizers using multiphoton microscopy

Eric A. Wachter; Craig Dees; Jay Harkins; Walter G. Fisher; Timothy Scott

doi:10.1117/12.470688

17 June 2002 Functional imaging of photosensitizers using multiphoton microscopy

Eric A. Wachter, Craig Dees, Jay Harkins, Walter G. Fisher, Timothy Scott

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470688
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Multiphoton imaging of the sub-cellular distribution of photosensitizers can provide important clues to their mechanism of action in tumors. We have used this tool to study distribution and pharmacology of photosensitizers in murine hepatoma tumor cells dosed with various photosensitizers. Upon photoactivation, Rose Bengal yields nearly immediate photolytic release of lysomal enzymes, resulting in catastrophic cell destruction within 5-30 minutes. Such marked response is quite different than that observed with other photosensitizer agents, and is consistent with in vivo studies illustrating that Rose Bengal is capable of causing extremely rapid destruction of treated tumors.

Citation Download Citation

Eric A. Wachter, Craig Dees, Jay Harkins, Walter G. Fisher, and Timothy Scott "Functional imaging of photosensitizers using multiphoton microscopy", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470688

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