Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques

[+] Author Affiliations
Kjell Carlsson, Johan Philip

Royal Institute of Technology (Sweden)

Proc. SPIE 4622, Optical Diagnostics of Living Cells V, 70 (May 28, 2002); doi:10.1117/12.468362
Text Size: A A A
From Conference Volume 4622

  • Optical Diagnostics of Living Cells V
  • Daniel L. Farkas; Robert C. Leif
  • San Jose, CA | January 19, 2002

abstract

Using Monte-Carlo methods, we have investigated the signal- to-noise ratio obtainable for different fluorescence lifetime imaging methods. Quantum noise limited performance and mono-exponential decays were assumed. We have also investigated the importance of parameter choice and implementation for the different methods. In addition, our simulations were in many cases compared with analytical theoretical investigations. The results from the simulations proved to be in good agreement with the theoretical results. It was found that all the investigated lifetime imaging methods have the potential to produce a high signal-to-noise ratio, but careful attention must be paid to implementation method and parameter choice in order to get optimal results.

© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Kjell Carlsson and Johan Philip
"Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, 70 (May 28, 2002); doi:10.1117/12.468362; http://dx.doi.org/10.1117/12.468362


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.