Measurement of adhesive forces between bacteria and protein-coated surfaces using optical tweezers

Kathryn H. Simpson; Gabriela Bowden; Magnus Hook; Bahman Anvari

doi:10.1117/12.468335

28 May 2002 Measurement of adhesive forces between bacteria and protein-coated surfaces using optical tweezers

Kathryn H. Simpson, Gabriela Bowden, Magnus Hook, Bahman Anvari

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468335
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Bacterial adhesion is a primary cause of failure in implanted medical devices. Bacteria commonly found in device-related infections, such as S. aureus, have multiple cell surface adhesins which mediate specific adhesion to molecules found in extracellular matrix and blood plasma. Adhesins recognizing fibrinogen, fibronectin, collagen, and elastin molecules have been isolated in S. aureus. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. Various concentrations of fibronectin, fibrinogen, or whole plasma were immobilized onto 10-micrometers diameter polystyrene microspheres. We optically trapped a bacterium with a titanium-sapphire laser tuned to 830 nm and contacted the cell with a coated bead. We determined the minimum force necessary to separate the cell and bead. For beads coated with fibronectin and fibrinogen, detachment force values occurred as approximate integer multiples of an estimated single bond detachment force. With plasma-coated beads, only cells lacking the fibrinogen adhesin could be detached; therefore, we believe that either this adhesin is prevalent on wilde-type cells, or it is preferentially adsorbed onto the beads. Additionally, the whole plasma detachment forces appeared random; therefore, we believe that many adhesins participate in boding to plasma.

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Kathryn H. Simpson, Gabriela Bowden, Magnus Hook, and Bahman Anvari "Measurement of adhesive forces between bacteria and protein-coated surfaces using optical tweezers", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468335

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KEYWORDS

Optical tweezers

Plasma

Molecules

Bacteria

Adhesives

Calibration

Natural surfaces

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