Trapping and tracking in the presence of phase disturbances

Peter C. Seitz; Ernst H. K. Stelzer; Alexander Rohrbach

doi:10.1117/12.616488

18 August 2005 Trapping and tracking in the presence of phase disturbances

Peter C. Seitz, Ernst H. K. Stelzer, Alexander Rohrbach

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 593005 (2005) https://doi.org/10.1117/12.616488
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

Functionalized surfaces can affect (bio-) chemical reactions and control spatially the affinity for various binding partners. By either distributing specific binding points, by using biological reconstituted systems on the surface or by investigating whole cells, a surface phase relief is introduced. In the proximity of a trapped particle, these surface scatterers will both disturb the optical trap and the position tracking signal by changing the wave front of the trapping laser. We investigate the influence of an additional scatterer on trapping force, detection sensitivity and local viscous drag by scanning an optically trapped bead (probe) across a structured surface. Using a photonic force microscope, the probe's fluctuation traces are recorded interferometrically in three dimensions with nm precision and at scan rates of several hundred kilohertz with a quadrant photodiode. The phase disturbance located underneath the optical trap alters the interferometric probe position signals and can lead to incorrect interaction measurements. We propose and test a procedure to correct for the phase disturbance of the surface structure. In a roll over experiment, where one nano-sphere rolls over another, we prove the applicability of our phase correction approach. In addition we investigate the influence of small gold dots on the coverslip on trapping parameters which are relevant for specific interaction measurements in biotechnology.

Citation Download Citation

Peter C. Seitz, Ernst H. K. Stelzer, and Alexander Rohrbach "Trapping and tracking in the presence of phase disturbances", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593005 (18 August 2005); https://doi.org/10.1117/12.616488

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical tweezers

Optical spheres

Particles

Gold

Diffusion

Proteins

Light scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years