We present an alternative approach to image and analyze a large amount of optically-bound particles without using microscope objective lenses. The polystyrene spheres were trapped and patterned in the one dimensional optical potential traps formed by two counter-propagating laser beams. Diffraction patterns from such polystyrene sphere assembly were studied and the result are reported. This method offers a significantly larger field of view and faster analysis over the conventional high-power microscope-based imaging technique. The applications of such technique to investigate the statistic (macroscopic) behaviors of a large number of microscopic particles trapped in the optical field were proposed.© (2005) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.