CellTracks: Cell analysis system for rare cell detection

Michael T. Kagan; Michael N. Trainer; Teresa Bendele; Chandra Rao; Allen Horton; Arjan G.J. Tibbe; Jan Greve; Leon W. M. M. Terstappen

doi:10.1117/12.469792

4 June 2002 CellTracks: Cell analysis system for rare cell detection

Michael T. Kagan, Michael N. Trainer, Teresa Bendele, Chandra Rao, Allen Horton, Arjan G.J. Tibbe, Jan Greve, Leon W. M. M. Terstappen

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Proceedings Volume 4625, Clinical Diagnostic Systems: Technologies and Instrumentation; (2002) https://doi.org/10.1117/12.469792
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The CellTracks system is a Compact Disk-based cell analyzer that, similar to flow cytometry, differentiates cells that are aligned while passing through focused laser beams. In CellTracks, only immuno-magnetically labeled cells are aligned and remain in position for further analysis. This feature is important in those cases were the cells are relatively infrequent. Epithelium derived tumor cells in peripheral blood are extremely rare but can be present in the blood of cancer patients. The certainty that an event present in a gate is, indeed, an epithelial cell with the assumed characteristics diminishes with the number of events in the analysis gate. Additional and preferably independent information on the individual events aids in the correct classification of the event as an epithelium derived tumor cell. Epithelial cells are immuno-magnetically selected from 7.5 mL of blood and magnetically aligned in the sample chamber between a series of parallel thin film nickel lines. The CD head scans along all nickel lines and captures the fluorescence signals of the objects between the lines. Objects that immuno-phenotypically classify as epithelial tumor cells are revisited for imaging to determine if the identified objects indeed classify as epithelial tumors cells or as debris derived from epithelial cells.

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Michael T. Kagan, Michael N. Trainer, Teresa Bendele, Chandra Rao, Allen Horton, Arjan G.J. Tibbe, Jan Greve, and Leon W. M. M. Terstappen "CellTracks: Cell analysis system for rare cell detection", Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); https://doi.org/10.1117/12.469792

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